Perform under hood.

Do not use this procedure with tissue that has been stained with fluorescent dye.

Will need:

250 mL DI water

250 mL 50% ethanol (50% 200 proof ethanol: 50% part DI water)

250 mL 75% ethanol (75% 200 proof ethanol: 25% part DI water)

250 mL 95% ethanol (95% 200 proof ethanol: 5% part DI water)

500 mL 100% ethanol (200 proof)

500 mL citrosolv

1. Immerse slides (in slide boat with metal handle) in DI water for 30 sec.
2. Immerse slides in 50% ethanol for 5 min.
3. Immerse slides in 75% ethanol for 5 min.
4. Immerse slides in 95% ethanol for 5 min.
5. Immerse slides in 100% ethanol for 5 min.
6. Immerse slides in 100% ethanol for 5 min.
7. Immerse slides in citrosolv (100%) for 5 min.
8. Immerse slides in citrosolv (100%) for 5 min.

Slides are ready to mount