NE : Norepinephrine (Arterenol)

DOPAC : 3, 4-dihydroxyphenylacetic acid

DOPAMINE: 3-hydroxytyramine

5-HIAA : 5-hydroxy-3-indoleacetic acid

HVA : Homovanilic acid (4-hydroxy-3-methoxyphenylacetic acid)

5-HT : 5-hydroxytryptamine (serotonin)

For 1 mg/ml concentration of individual STOCK A solutions:

NE                   9.45 mg/ 5 ml of HPLC grade water

DOPAC          5.00 mg/ 5 ml              “

DA                  5.00 mg/ 4.03 ml         “

5-HIAA          5.00 mg/ 5 ml              “

HVA               5.00 mg/ 5 ml              “

5-HT               5.00 mg/ 2.17 ml         “

5-HTOL          5.00 mg/ 5 ml              “

For 1 μg/ml concentration of individual STOCK B solutions:

5 μl of STOCK A/ 4995 μl of HPLC-grade water

For working solution:

80 μl of STOCK B/ 4920 μl of HPLC grade water to get a 16 pg/μl concentration

40 μl of STOCK B/ 4960 μl of HPLC grade water to get an 8 pg/μl concentration

20 μl of STOCK B/ 4980 μl of HPLC grade water to get a 4 pg/μl concentration

 

And so on…