Developer solution= 1 part concentrated developer solution + 4 parts DI water (e.g. 50 mL developer + 200 mL water)

Fixer solution= 1 part concentrated fixer solution + 4 parts DI water (e.g. 50 mL fixer + 200 mL water)

After exposing autorad to ECL-enhanced membranes incubate the autorad in the following solutions, in a flat open container/tub:

1. Incubate autorad in developer solution for 2 min
2. Incubate/rinse in DI water for 30 sec
3. Incubate autorad in fixer solution for 2 min
4. Incubate/rinse in DI water for 30 sec
5. Hang-up to airdry

Parts:

Concentrated developer, from Fisher, Cat. # 05-728-104 (Kodak part number 190-0943)

Concentrated fixer, from Fisher, Cat. # 05-728-112 (Kodak part number 190-1875)