{"id":153,"date":"2012-01-10T07:46:00","date_gmt":"2012-01-10T07:46:00","guid":{"rendered":"http:\/\/labs.la.utexas.edu\/dominguez\/?p=153"},"modified":"2016-05-04T22:25:37","modified_gmt":"2016-05-04T22:25:37","slug":"recipe-biogenic-amine-standards","status":"publish","type":"post","link":"https:\/\/labs.la.utexas.edu\/dominguez\/2012\/01\/10\/recipe-biogenic-amine-standards\/","title":{"rendered":"RECIPE: Biogenic Amine Standards"},"content":{"rendered":"

NE : Norepinephrine (Arterenol)<\/p>\n

DOPAC : 3, 4-dihydroxyphenylacetic acid<\/p>\n

DOPAMINE: 3-hydroxytyramine<\/p>\n

5-HIAA : 5-hydroxy-3-indoleacetic acid<\/p>\n

HVA : Homovanilic acid (4-hydroxy-3-methoxyphenylacetic acid)<\/p>\n

5-HT : 5-hydroxytryptamine (serotonin)<\/p>\n

For 1 mg\/ml concentration of individual STOCK A<\/u><\/strong> solutions:<\/p>\n

NE\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 9.45 mg\/ 5 ml of HPLC grade water<\/p>\n

DOPAC\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 5 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0 \u201c<\/p>\n

DA\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 4.03 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u201c<\/p>\n

5-HIAA\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 5 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u201c<\/p>\n

HVA\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 5 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u201c<\/p>\n

5-HT\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 2.17 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u201c<\/p>\n

5-HTOL\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 5 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u201c<\/p>\n

For 1 \u03bcg\/ml concentration of individual STOCK B<\/u><\/strong> solutions:<\/p>\n

5 \u03bcl of STOCK A\/ 4995 \u03bcl of HPLC-grade water<\/p>\n

For working solution:<\/p>\n

80 \u03bcl of STOCK B\/ 4920 \u03bcl of HPLC grade water to get a 16 pg\/\u03bcl concentration<\/p>\n

40 \u03bcl of STOCK B\/ 4960 \u03bcl of HPLC grade water to get an 8 pg\/\u03bcl concentration<\/p>\n

20 \u03bcl of STOCK B\/ 4980 \u03bcl of HPLC grade water to get a 4 pg\/\u03bcl concentration<\/p>\n

 <\/p>\n

And so on\u2026<\/p>\n","protected":false},"excerpt":{"rendered":"

NE : Norepinephrine (Arterenol) DOPAC : 3, 4-dihydroxyphenylacetic acid DOPAMINE: 3-hydroxytyramine 5-HIAA : 5-hydroxy-3-indoleacetic acid HVA : Homovanilic acid (4-hydroxy-3-methoxyphenylacetic acid) 5-HT : 5-hydroxytryptamine (serotonin) For 1 mg\/ml concentration of individual STOCK A solutions: NE\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 9.45 mg\/ 5 ml of HPLC grade water DOPAC\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 5 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0 \u201c DA\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 4.03 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 […]<\/p>\n","protected":false},"author":103,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[4],"tags":[],"class_list":["post-153","post","type-post","status-publish","format-standard","hentry","category-recipe","entry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"site-graphic":false},"uagb_author_info":{"display_name":"djh2697","author_link":"https:\/\/labs.la.utexas.edu\/dominguez\/author\/djh2697\/"},"uagb_comment_info":0,"uagb_excerpt":"NE : Norepinephrine (Arterenol) DOPAC : 3, 4-dihydroxyphenylacetic acid DOPAMINE: 3-hydroxytyramine 5-HIAA : 5-hydroxy-3-indoleacetic acid HVA : Homovanilic acid (4-hydroxy-3-methoxyphenylacetic acid) 5-HT : 5-hydroxytryptamine (serotonin) For 1 mg\/ml concentration of individual STOCK A solutions: NE\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 9.45 mg\/ 5 ml of HPLC grade water DOPAC\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 5 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0 \u201c DA\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 5.00 mg\/ 4.03 ml\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0…","_links":{"self":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/153","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/users\/103"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/comments?post=153"}],"version-history":[{"count":1,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/153\/revisions"}],"predecessor-version":[{"id":154,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/153\/revisions\/154"}],"wp:attachment":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/media?parent=153"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/categories?post=153"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/tags?post=153"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}