{"id":149,"date":"2012-01-10T07:49:23","date_gmt":"2012-01-10T07:49:23","guid":{"rendered":"http:\/\/labs.la.utexas.edu\/dominguez\/?p=149"},"modified":"2016-05-04T22:25:27","modified_gmt":"2016-05-04T22:25:27","slug":"protocol-developing-autorad-film-in-darkroom-for-western-immunoblotting","status":"publish","type":"post","link":"https:\/\/labs.la.utexas.edu\/dominguez\/2012\/01\/10\/protocol-developing-autorad-film-in-darkroom-for-western-immunoblotting\/","title":{"rendered":"PROTOCOL: Developing Autorad film in darkroom for Western immunoblotting"},"content":{"rendered":"

Developer solution<\/u><\/em>= 1 part concentrated developer solution + 4 parts DI water (e.g. 50 mL developer + 200 mL water)<\/p>\n

Fixer solution<\/u><\/em>= 1 part concentrated fixer solution + 4 parts DI water (e.g. 50 mL fixer + 200 mL water)<\/p>\n

After exposing autorad to ECL-enhanced membranes incubate the autorad in the following solutions, in a flat open container\/tub:<\/p>\n

    \n
  1. Incubate autorad in developer solution for 2 min<\/li>\n
  2. Incubate\/rinse in DI water for 30 sec<\/li>\n
  3. Incubate autorad in fixer solution for 2 min<\/li>\n
  4. Incubate\/rinse in DI water for 30 sec<\/li>\n
  5. Hang-up to airdry<\/li>\n<\/ol>\n

    Parts:<\/p>\n

    Concentrated developer, from Fisher, Cat. # 05-728-104 (Kodak part number 190-0943)<\/p>\n

    Concentrated fixer, from Fisher, Cat. # 05-728-112 (Kodak part number 190-1875)<\/p>\n","protected":false},"excerpt":{"rendered":"

    Developer solution= 1 part concentrated developer solution + 4 parts DI water (e.g. 50 mL developer + 200 mL water) Fixer solution= 1 part concentrated fixer solution + 4 parts DI water (e.g. 50 mL fixer + 200 mL water) After exposing autorad to ECL-enhanced membranes incubate the autorad in the following solutions, in a […]<\/p>\n","protected":false},"author":103,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[3],"tags":[],"class_list":["post-149","post","type-post","status-publish","format-standard","hentry","category-protocol","entry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"site-graphic":false},"uagb_author_info":{"display_name":"djh2697","author_link":"https:\/\/labs.la.utexas.edu\/dominguez\/author\/djh2697\/"},"uagb_comment_info":0,"uagb_excerpt":"Developer solution= 1 part concentrated developer solution + 4 parts DI water (e.g. 50 mL developer + 200 mL water) Fixer solution= 1 part concentrated fixer solution + 4 parts DI water (e.g. 50 mL fixer + 200 mL water) After exposing autorad to ECL-enhanced membranes incubate the autorad in the following solutions, in a…","_links":{"self":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/149","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/users\/103"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/comments?post=149"}],"version-history":[{"count":1,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/149\/revisions"}],"predecessor-version":[{"id":150,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/149\/revisions\/150"}],"wp:attachment":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/media?parent=149"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/categories?post=149"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/tags?post=149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}