{"id":145,"date":"2012-01-12T07:59:47","date_gmt":"2012-01-12T07:59:47","guid":{"rendered":"http:\/\/labs.la.utexas.edu\/dominguez\/?p=145"},"modified":"2016-05-04T22:25:19","modified_gmt":"2016-05-04T22:25:19","slug":"protocol-dehydrating-mounted-slides","status":"publish","type":"post","link":"https:\/\/labs.la.utexas.edu\/dominguez\/2012\/01\/12\/protocol-dehydrating-mounted-slides\/","title":{"rendered":"PROTOCOL: Dehydrating mounted slides"},"content":{"rendered":"<p>Perform under hood.<\/p>\n<p>Do not use this procedure with tissue that has been stained with fluorescent dye.<\/p>\n<p>Will need:<\/p>\n<p>250 mL DI water<\/p>\n<p>250 mL 50% ethanol (50% 200 proof ethanol: 50% part DI water)<\/p>\n<p>250 mL 75% ethanol (75% 200 proof ethanol: 25% part DI water)<\/p>\n<p>250 mL 95% ethanol (95% 200 proof ethanol: 5% part DI water)<\/p>\n<p>500 mL 100% ethanol (200 proof)<\/p>\n<p>500 mL citrosolv<\/p>\n<ol>\n<li>Immerse slides (in slide boat with metal handle) in DI water for 30 sec.<\/li>\n<li>Immerse slides in 50% ethanol for 5 min.<\/li>\n<li>Immerse slides in 75% ethanol for 5 min.<\/li>\n<li>Immerse slides in 95% ethanol for 5 min.<\/li>\n<li>Immerse slides in 100% ethanol for 5 min.<\/li>\n<li>Immerse slides in 100% ethanol for 5 min.<\/li>\n<li>Immerse slides in citrosolv (100%) for 5 min.<\/li>\n<li>Immerse slides in citrosolv (100%) for 5 min.<\/li>\n<\/ol>\n<p>Slides are ready to mount<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Perform under hood. Do not use this procedure with tissue that has been stained with fluorescent dye. Will need: 250 mL DI water 250 mL 50% ethanol (50% 200 proof ethanol: 50% part DI water) 250 mL 75% ethanol (75% 200 proof ethanol: 25% part DI water) 250 mL 95% ethanol (95% 200 proof ethanol: [&hellip;]<\/p>\n","protected":false},"author":103,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[3],"tags":[],"class_list":["post-145","post","type-post","status-publish","format-standard","hentry","category-protocol","entry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"site-graphic":false},"uagb_author_info":{"display_name":"djh2697","author_link":"https:\/\/labs.la.utexas.edu\/dominguez\/author\/djh2697\/"},"uagb_comment_info":0,"uagb_excerpt":"Perform under hood. Do not use this procedure with tissue that has been stained with fluorescent dye. Will need: 250 mL DI water 250 mL 50% ethanol (50% 200 proof ethanol: 50% part DI water) 250 mL 75% ethanol (75% 200 proof ethanol: 25% part DI water) 250 mL 95% ethanol (95% 200 proof ethanol:&hellip;","_links":{"self":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/145","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/users\/103"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/comments?post=145"}],"version-history":[{"count":1,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/145\/revisions"}],"predecessor-version":[{"id":146,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/145\/revisions\/146"}],"wp:attachment":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/media?parent=145"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/categories?post=145"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/tags?post=145"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}