{"id":143,"date":"2012-01-10T08:01:53","date_gmt":"2012-01-10T08:01:53","guid":{"rendered":"http:\/\/labs.la.utexas.edu\/dominguez\/?p=143"},"modified":"2016-05-04T22:26:00","modified_gmt":"2016-05-04T22:26:00","slug":"recipe-cryoprotectant","status":"publish","type":"post","link":"https:\/\/labs.la.utexas.edu\/dominguez\/2012\/01\/10\/recipe-cryoprotectant\/","title":{"rendered":"RECIPE: Cryoprotectant"},"content":{"rendered":"

350 ml 0.1 M PB solution (pH 7.35)<\/p>\n

150 ml ethylene glycol<\/p>\n

100 \u03bcg sodium azide<\/p>\n

150 g sucrose<\/p>\n

Final cryoprotectant concentration will be: 30% ethylene glycol, 30% sucrose, 0.00002% sodium azide, in 0.1 M PB (pH 7.35).\u00a0 Prepare this solution under the hood.<\/p>\n

    \n
  1. Pour 250 ml of 0.1 M PB into 1 L flask.<\/li>\n
  2. Pour 150 ml of ethylene glycol into the flask.<\/li>\n
  3. Add 100 \u03bcg sodium azide.<\/li>\n
  4. Stir for 30 min<\/li>\n
  5. Add 150 g of sucrose<\/li>\n
  6. Stir for 2 hr<\/li>\n
  7. Bring volume up to 500 ml with 0.1 M PB<\/li>\n
  8. Store cryoprotectant solution in refrigerator (+4\u00baC)<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    350 ml 0.1 M PB solution (pH 7.35) 150 ml ethylene glycol 100 \u03bcg sodium azide 150 g sucrose Final cryoprotectant concentration will be: 30% ethylene glycol, 30% sucrose, 0.00002% sodium azide, in 0.1 M PB (pH 7.35).\u00a0 Prepare this solution under the hood. Pour 250 ml of 0.1 M PB into 1 L flask. […]<\/p>\n","protected":false},"author":103,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[4],"tags":[],"class_list":["post-143","post","type-post","status-publish","format-standard","hentry","category-recipe","entry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"site-graphic":false},"uagb_author_info":{"display_name":"djh2697","author_link":"https:\/\/labs.la.utexas.edu\/dominguez\/author\/djh2697\/"},"uagb_comment_info":1,"uagb_excerpt":"350 ml 0.1 M PB solution (pH 7.35) 150 ml ethylene glycol 100 \u03bcg sodium azide 150 g sucrose Final cryoprotectant concentration will be: 30% ethylene glycol, 30% sucrose, 0.00002% sodium azide, in 0.1 M PB (pH 7.35).\u00a0 Prepare this solution under the hood. Pour 250 ml of 0.1 M PB into 1 L flask.…","_links":{"self":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/143","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/users\/103"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/comments?post=143"}],"version-history":[{"count":1,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/143\/revisions"}],"predecessor-version":[{"id":144,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/143\/revisions\/144"}],"wp:attachment":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/media?parent=143"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/categories?post=143"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/tags?post=143"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}