{"id":140,"date":"2012-01-10T08:02:49","date_gmt":"2012-01-10T08:02:49","guid":{"rendered":"http:\/\/labs.la.utexas.edu\/dominguez\/?p=140"},"modified":"2016-05-04T22:26:15","modified_gmt":"2016-05-04T22:26:15","slug":"recipe-gels-for-western-immunoblots","status":"publish","type":"post","link":"https:\/\/labs.la.utexas.edu\/dominguez\/2012\/01\/10\/recipe-gels-for-western-immunoblots\/","title":{"rendered":"RECIPE: Gels for Western immunoblots"},"content":{"rendered":"

<\/h1>\n\n\n\n\n\n\n\n\n\n\n
MATERIALS<\/td>\n8%<\/td>\n10%<\/td>\n12.5%<\/td>\n15%<\/td>\n17.5%<\/td>\n<\/tr>\n
29:1 Acrylamide<\/td>\n2.2 mL<\/td>\n2.7<\/td>\n3.3<\/td>\n4.0<\/td>\n4.67<\/td>\n<\/tr>\n
4X Lower Tris<\/td>\n2.0 mL<\/td>\n2<\/td>\n2<\/td>\n2<\/td>\n2<\/td>\n<\/tr>\n
ddH2O<\/td>\n3.8 mL<\/td>\n3.25<\/td>\n2.65<\/td>\n1.95<\/td>\n1.3<\/td>\n<\/tr>\n
APS<\/td>\n40 uL<\/td>\n40 uL<\/td>\n40 uL<\/td>\n40 uL<\/td>\n40 uL<\/td>\n<\/tr>\n
TEMED<\/td>\n5 uL<\/td>\n5 uL<\/td>\n5 uL<\/td>\n5 uL<\/td>\n5 uL<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Total Vol:<\/td>\n8 mL<\/td>\n8 mL<\/td>\n8 mL<\/td>\n8 mL<\/td>\n8 mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Separation gel:<\/p>\n

Stacking gel:<\/p>\n\n\n\n\n\n\n\n\n\n\n
MATERIALS<\/td>\n<\/td>\n<\/tr>\n
29:1 Acrylamide<\/td>\n0.7 mL<\/td>\n<\/tr>\n
4X Upper Tris<\/td>\n1 mL<\/td>\n<\/tr>\n
ddH2O<\/td>\n2.3 mL<\/td>\n<\/tr>\n
APS<\/td>\n20 uL<\/td>\n<\/tr>\n
TEMED<\/td>\n2.5 uL<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n
Total Vol<\/td>\n4 mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Note: These recipes are for single gel, for double gels simply double everything.<\/p>\n","protected":false},"excerpt":{"rendered":"

MATERIALS 8% 10% 12.5% 15% 17.5% 29:1 Acrylamide 2.2 mL 2.7 3.3 4.0 4.67 4X Lower Tris 2.0 mL 2 2 2 2 ddH2O 3.8 mL 3.25 2.65 1.95 1.3 APS 40 uL 40 uL 40 uL 40 uL 40 uL TEMED 5 uL 5 uL 5 uL 5 uL 5 uL Total Vol: 8 […]<\/p>\n","protected":false},"author":103,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[4],"tags":[],"class_list":["post-140","post","type-post","status-publish","format-standard","hentry","category-recipe","entry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"site-graphic":false},"uagb_author_info":{"display_name":"djh2697","author_link":"https:\/\/labs.la.utexas.edu\/dominguez\/author\/djh2697\/"},"uagb_comment_info":0,"uagb_excerpt":"MATERIALS 8% 10% 12.5% 15% 17.5% 29:1 Acrylamide 2.2 mL 2.7 3.3 4.0 4.67 4X Lower Tris 2.0 mL 2 2 2 2 ddH2O 3.8 mL 3.25 2.65 1.95 1.3 APS 40 uL 40 uL 40 uL 40 uL 40 uL TEMED 5 uL 5 uL 5 uL 5 uL 5 uL Total Vol: 8…","_links":{"self":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/140","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/users\/103"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/comments?post=140"}],"version-history":[{"count":1,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/140\/revisions"}],"predecessor-version":[{"id":141,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/140\/revisions\/141"}],"wp:attachment":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/media?parent=140"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/categories?post=140"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/tags?post=140"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}