
{"id":135,"date":"2012-01-10T08:08:14","date_gmt":"2012-01-10T08:08:14","guid":{"rendered":"http:\/\/labs.la.utexas.edu\/dominguez\/?p=135"},"modified":"2016-05-04T22:26:22","modified_gmt":"2016-05-04T22:26:22","slug":"recipe-mobile-phase-for-amino-acids","status":"publish","type":"post","link":"https:\/\/labs.la.utexas.edu\/dominguez\/2012\/01\/10\/recipe-mobile-phase-for-amino-acids\/","title":{"rendered":"RECIPE: Mobile phase for amino acids"},"content":{"rendered":"<p>12.06 g of sodium phosphate dibasic<\/p>\n<p>50.25 mg (0.05025 g) of EDTA (Ethylenediaminetetraacetic acid disodium salt dehydrate)<\/p>\n<p>150 mL methanol<\/p>\n<p>in 850 mL HPLC water<\/p>\n<p>pH 6.50<\/p>\n<p>[use HPLC grade O-phosphoric acid (85%) or 6.25 N sodium hydroxide to pH the mobile phase; Fisher Cat # A260-500]<\/p>\n<p>Catalog numbers from suppliers:<\/p>\n<p>HPLC Water, Fisher<\/p>\n<p>HPLC Methanol, Fisher Cat # A452-4<\/p>\n<p>Ethylenediaminetetraacetic acid disodium salt dehydrate, Sigma Cat # ED2SS<\/p>\n<p>O-phosphoric acid (85%), Fisher Cat # A260-500<\/p>\n<p>6.25 N sodium hydroxide, Fisher Cat # SS263-500<\/p>\n<p>Sodium phosphate dibasic, Fisher Cat # S374-3<\/p>\n","protected":false},"excerpt":{"rendered":"<p>12.06 g of sodium phosphate dibasic 50.25 mg (0.05025 g) of EDTA (Ethylenediaminetetraacetic acid disodium salt dehydrate) 150 mL methanol in 850 mL HPLC water pH 6.50 [use HPLC grade O-phosphoric acid (85%) or 6.25 N sodium hydroxide to pH the mobile phase; Fisher Cat # A260-500] Catalog numbers from suppliers: HPLC Water, Fisher HPLC [&hellip;]<\/p>\n","protected":false},"author":103,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[4],"tags":[],"class_list":["post-135","post","type-post","status-publish","format-standard","hentry","category-recipe","entry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"site-graphic":false},"uagb_author_info":{"display_name":"djh2697","author_link":"https:\/\/labs.la.utexas.edu\/dominguez\/author\/djh2697\/"},"uagb_comment_info":0,"uagb_excerpt":"12.06 g of sodium phosphate dibasic 50.25 mg (0.05025 g) of EDTA (Ethylenediaminetetraacetic acid disodium salt dehydrate) 150 mL methanol in 850 mL HPLC water pH 6.50 [use HPLC grade O-phosphoric acid (85%) or 6.25 N sodium hydroxide to pH the mobile phase; Fisher Cat # A260-500] Catalog numbers from suppliers: HPLC Water, Fisher HPLC&hellip;","_links":{"self":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/135","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/users\/103"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/comments?post=135"}],"version-history":[{"count":1,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/135\/revisions"}],"predecessor-version":[{"id":136,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/135\/revisions\/136"}],"wp:attachment":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/media?parent=135"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/categories?post=135"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/tags?post=135"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}