960 ml HPLC grade water<\/p>\n
40 ml HPLC grade methanol<\/p>\n
7.4 g citric acid anhydrous<\/p>\n
7.4 g sodium acetate anhydrous<\/p>\n
0.16 g EDTA (Ethylenediaminetetraacetic acid disodium salt dehydrate)<\/p>\n
0.08 g OSA (1-Octanesulfonic Acid Sodium Salt)<\/p>\n
[use HPLC grade O-phosphoric acid (85%) or 6.25 N sodium hydroxide to pH the mobile phase; Fisher Cat # A260-500]<\/p>\n
Catalog numbers from suppliers:<\/p>\n
HPLC Water, Fisher<\/p>\n
HPLC Methanol, Fisher Cat # A452-4<\/p>\n
Citric acid anhydrous, Fluka Cat # 27487<\/p>\n
Sodium acetate anhydrous, Fisher Cat # S209-500<\/p>\n
Ethylenediaminetetraacetic acid disodium salt dehydrate, Sigma Cat # ED2SS<\/p>\n
1-Octanesulfonic Acid Sodium Salt, Sigma Cat # O-0133<\/p>\n
O-phosphoric acid (85%), Fisher Cat # A260-500<\/p>\n
6.25 N sodium hydroxide, Fisher Cat # SS263-500<\/p>\n","protected":false},"excerpt":{"rendered":"
960 ml HPLC grade water 40 ml HPLC grade methanol 7.4 g citric acid anhydrous 7.4 g sodium acetate anhydrous 0.16 g EDTA (Ethylenediaminetetraacetic acid disodium salt dehydrate) 0.08 g OSA (1-Octanesulfonic Acid Sodium Salt) [use HPLC grade O-phosphoric acid (85%) or 6.25 N sodium hydroxide to pH the mobile phase; Fisher Cat # A260-500] […]<\/p>\n","protected":false},"author":103,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[4],"tags":[],"class_list":["post-129","post","type-post","status-publish","format-standard","hentry","category-recipe","entry"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"site-graphic":false},"uagb_author_info":{"display_name":"djh2697","author_link":"https:\/\/labs.la.utexas.edu\/dominguez\/author\/djh2697\/"},"uagb_comment_info":0,"uagb_excerpt":"960 ml HPLC grade water 40 ml HPLC grade methanol 7.4 g citric acid anhydrous 7.4 g sodium acetate anhydrous 0.16 g EDTA (Ethylenediaminetetraacetic acid disodium salt dehydrate) 0.08 g OSA (1-Octanesulfonic Acid Sodium Salt) [use HPLC grade O-phosphoric acid (85%) or 6.25 N sodium hydroxide to pH the mobile phase; Fisher Cat # A260-500]…","_links":{"self":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/129","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/users\/103"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/comments?post=129"}],"version-history":[{"count":1,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/129\/revisions"}],"predecessor-version":[{"id":130,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/posts\/129\/revisions\/130"}],"wp:attachment":[{"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/media?parent=129"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/categories?post=129"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/labs.la.utexas.edu\/dominguez\/wp-json\/wp\/v2\/tags?post=129"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}