960 ml HPLC grade water

40 ml HPLC grade methanol

7.4 g citric acid anhydrous

7.4 g sodium acetate anhydrous

0.16 g EDTA (Ethylenediaminetetraacetic acid disodium salt dehydrate)

0.08 g OSA (1-Octanesulfonic Acid Sodium Salt)

[use HPLC grade O-phosphoric acid (85%) or 6.25 N sodium hydroxide to pH the mobile phase; Fisher Cat # A260-500]

Catalog numbers from suppliers:

HPLC Water, Fisher

HPLC Methanol, Fisher Cat # A452-4

Citric acid anhydrous, Fluka Cat # 27487

Sodium acetate anhydrous, Fisher Cat # S209-500

Ethylenediaminetetraacetic acid disodium salt dehydrate, Sigma Cat # ED2SS

1-Octanesulfonic Acid Sodium Salt, Sigma Cat # O-0133

O-phosphoric acid (85%), Fisher Cat # A260-500

6.25 N sodium hydroxide, Fisher Cat # SS263-500