Cresyl violet staining for mounted slides.
- 95% ethanol …………………………… 10 min
- 70% ethanol …………………………… 1 min
- 50% ethanol …………………………… 1 min
- dH2O …………………………… 2 min
- dH2O …………………………… 1 min
- Cresyl violet stain*…………………………… 10 min
- dH2O …………………………… 2 min
- 50% ethanol …………………………… 1 min
- 70% acid ethanol+ ………………………… 2 min
- 95% ethanol …………………………… 2 min
- 95% ethanol …………………………… 30 seconds
- 100% ethanol …………………………… 1 min
- Xylene …………………………… 3 min
- Air dry and coverslip.
* Cresyl violet stain: add 1.25 g cresyl violet acetate and 0.75 mL glacial acetic acid to 250 mL of warm dH2O, then cool and filter.
+ 70% acid ethanol: 2 mL glacial acetic acid in 200 mL 70% ethanol.
Neuroendocrinology and Motivation Lab
